Your plan is very weak. You cannot just loop one BluRay score with the sub in each location and get an accurate understanding of how each location sounds different. First off, you absolutely MUST calibrate the sub after moving it because one location could be 6dB SPL louder than the previous dueto acoustics while performing much worse in terms of balance, decay, and standing waves. You could easily choose the worst location due to the higher SPL without realizing the bigger issues.
If you don't already own a calibration solution (TrueRTA, Mic, Preamp), then I would strongly encourage you to look into some sort of solution - even a cheap SPL meter from Radio Shack and some downloaded test tones is a better choice than relying on your ears.
- Take the time to calibrate the sub when you move it.
- Listen to it for a day with a long list of different music and movie content.
- Take copious notes on your experience in each location.
- Make a chart of the key audible characteristics of the sound: Tightness vs. Bloat; Balance; Realism; Blending in with the rest of the sound; Extension; Impact; Decay; etc.
Then choose the best two locations and compare them again using the same criteria.
This could take a few weeks to get absolutely right, but it will be worth it in the long run.
Another option is to buy TrueRTA level 4 ($99), a calibrated mic ($39), a preamp ($49), a cable ($10), and a mic stand ($20) and use them properly to know for sure which location is most ideal in a matter of one evening. Personally, I would place the sub in the listening location at ear level then move the mic around to the various placement locations and quickly get an idea of which is best.